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. 2022 May 23:e221113. Online ahead of print. doi: 10.1001/jamaneurol.2022.1113

Figure 2. Axonal Phosphorylated Transactive Response DNA-Binding Protein 43 (pTDP-43)–Positive Accumulations in Intramuscular Nerve Bundles of Skeletal Muscle in the Postmortem Case-Control Study.

Figure 2.

A, D, Hematoxylin-eosin staining in axons of intramuscular nerve bundles showed no differences between patients with amyotrophic lateral sclerosis (ALS) and those without ALS except for the density of intramuscular nerves (original magnification: A, ×40; D, ×60). B, Immunohistochemical analysis with mouse monoclonal pTDP-43–positive accumulations in intramuscular nerve bundles (arrowheads) of patients with spontaneous ALS (SALS) (original magnification, ×40). E, Immunostaining with mouse monoclonal antibody against pTDP-43 in patients with non-ALS diseases did not reveal any abnormalities in intramuscular nerve bundles (original magnification, ×40). C, Immunohistochemical analysis with rabbit polyclonal pTDP-43 antibody also revealed pTDP-43–positive accumulations in intramuscular nerve bundles (arrowheads) of patients with SALS (original magnification, ×60). F, Immunostaining with rabbit polyclonal antibody against pTDP-43 in patients with non-ALS diseases did not reveal any abnormalities in intramuscular nerve bundles (original magnification, ×40). G, Summary of immunopositive nerve bundle detection in patients with autopsy-confirmed ALS. NA indicates not applicable.