Figure 11.

A) Summary of the binding assay data for NDI‐5 on nude and functionalized CPG supports. Bound ligand calculated as a difference from the unbound ligand and expressed as % of the amount initially loaded on the support. The errors associated with the % are within ±2 %. B) Representative merged images of immunofluorescence of BJ‐EHLT cancer cell line treated with NDI‐5; H2AX (DNA damage marker) spots are green, TRF1 (telomeric marker) spots are red and nuclei are blue. Enlarged views of Telomere Induced Foci (TIFs) are reported on the right smaller panels. C) Job plot analysis for the binding of NDI‐5 to tel26 G4. D) CD spectra of tel46 G4 in the absence and presence of increasing amounts of NDI‐5. Black arrows indicate intensity changes of the specific bands upon increasing NDI‐5 concentration. E) ITC binding isotherm for titration of tel46 G4 with NDI‐5. F) Binding mode of NDI‐5 to tel46 G4 obtained by molecular docking. A), B) and C) are adapted with permission from Ref. [66] under CC‐BY 4.0, Copyright 2020 by the authors; D), E) and F) are adapted with permission from Ref. [67] Copyright 2020 Elsevier.