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. 2022 Apr 28;17(5):1174–1183. doi: 10.1021/acschembio.2c00122

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© 2022 The Authors. Published by American Chemical Society

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Synthesis and characterization of pac-WOBE (3). (A) Reagents and conditions: (a) 5-(2-aminoethyl)-2-methoxyphenol, HOBt, EDC, 0 °C to rt, 43%; (b) 3-(but-3-yn-1-yl)-3-(2-iodoethyl)-3H-diazirine, K₂CO₃, 60 °C, 29%. (B) Neuro-2a cells were treated with 10 μM WOBE437 or vehicle and subsequently with 0.1 μM pac-WOBE (3) or vehicle, irradiated, and lysed, and proteomes were conjugated to Cy5-N₃ using CuAAC chemistry and analyzed by SDS-PAGE and in-gel fluorescence scanning. Coomassie served as a protein loading control. Arrows indicate WOBE437-competed targets.