Table 1. Bacterial Strains and Plasmids Used in This Study.
| name | description | reference |
|---|---|---|
| Bacterial strains | ||
| E. coli TOP10 | cloning strain | Invitrogen |
| E. coli sExpress | conjugation donor strain | (45) |
| C. autoethanogenum C24 | C. autoethanogenum DSM 10061 with genome-integrated lactose-inducible tcdR regulator | (46) |
| C. autoethanogenum C24 EG | C. autoethanogenum C24 carrying pMTL83251-EG and pMTL84151-fucO | this study |
| Plasmids | ||
| pMTL83251 | shuttle vector; pCB102 replicon; ermB | (56) |
| pMTL84151 | shuttle vector; pCD6 replicon; catP | (56) |
| pMTL83251-EG | pMTL83251 with the synthetic operon aceA-ghrA-aldA controlled by the PtdcB promoter | this study |
| pMTL84151-fucO | pMTL84151 with fucO controlled by the theophylline-inducible riboswitch47 | this study |