Table 2. EG Production at t = 96 h for the Two Transformants with Different Inducersa.
| transformants | conditions | OD600 | measured EG concentration (mM) | EG concentration normalized to OD (mM/OD600) | product (EG) yield (g/g) | % FBA |
|---|---|---|---|---|---|---|
| transformant 1 | –lact. −theo. | 2.27 | 7.43 | 3.28 | 0.020 | 2.46 |
| –lact. +theo. | 2.30 | 5.90 | 2.57 | 0.016 | 1.93 | |
| +lact. −theo. | 1.42 | 6.42 | 4.51 | 0.028 | 3.39 | |
| +lact. +theo. | 1.37 | 6.34 | 4.64 | 0.029 | 3.49 | |
| transformant 2 | –lact. −theo. | 2.42 | 7.61 | 3.15 | 0.020 | 2.36 |
| –lact. +theo. | 2.40 | 6.26 | 2.60 | 0.016 | 1.96 | |
| + lact. −theo. | 2.41 | 7.03 | 2.91 | 0.018 | 2.19 | |
| +lact. +theo. | 1.30 | 5.30 | 4.08 | 0.025 | 3.06 |
Product yield is also listed and represented as the percentage of the model-based FBA prediction. Lact = lactose; Theo = theophylline.