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. Author manuscript; available in PMC: 2022 May 24.
Published in final edited form as: Cell Rep. 2022 May 3;39(5):110767. doi: 10.1016/j.celrep.2022.110767

Figure 7. Regulation of HCMV replication by host eIF3d-responsive genes.

Figure 7.

(A) Total protein from mock- or HCMV-infected (TB40/E strain; MOI = 3) NHDFs treated with the indicated siRNA (non-silencing controlor eIF3d-specific siRNA #2) was collected at 72 hpi, fractionated by SDS-PAGE, and analyzed by immunoblotting using the antibodies shown. GAPDH provides a loading control.

(B) Viability of NHDFs transfected with siRNAs specific for the indicated gene (n = 3). Error bars indicate SEM.

(C–E) As in (A) except total protein from HCMV-infected (TB40/E strain; MOI = 0.1) NHDFs transfected with the specified siRNA was collected at 5 dpi.

(F–H) As in (C)–(E) except infectious virus was quantified from supernatants collected 5 dpi using a TCID50 assay (n = 3). Error bars indicate SEM. **p ≤ 0.01; ***p ≤ 0.001 by Student’s t test.