KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-eIF3a | Cell Signaling | cat# 3411S |
| anti-eIF3b | Bethyl | cat# A301-761A |
| anti-eIF3d | Proteintech | cat# 10219-1-AP |
| anti-eIF3e | Bethyl | cat# A302-985A |
| anti-eIF3g | Bethyl | cat# A301-757A |
| anti-eIF3h | Cell Signaling | cat# 3413S |
| anti-eIF3l | GeneTex | cat# GTX120119 |
| anti-4EBP1 | Bethyl | cat# A300-501A |
| anti-GAPDH | Cell Signaling | cat# 2118S |
| anti-IE1/IE2 | Millipore | cat# MAB810 |
| anti-UL44 | Virusys | cat# CA006 |
| anti-pp28 | Virusys | cat# CA004-100 |
| anti-UL57/ICP8 | Novus | cat# NB110-57369 |
| anti-DDX21 | Proteintech | cat# 10528-1-AP |
| anti-VCP | Proteintech | cat# 10736-1-AP |
| anti-SLC3A2/CD98 | Proteintech | cat# 15193-1-AP |
| anti-INPP4B | Cell Signaling | cat# 4039S |
| anti-RAD23A | Cell Signaling | cat# 24555S |
| anti-MLST8/GBL | Cell Signaling | cat# 3274S |
| anti-WIPI2 | Cell Signaling | cat# 8567S |
| anti-rabbit IgG HRP | GE Healthcare | cat# NA934-1ML |
| anti-mouse IgG HRP | GE Healthcare | cat# NA931-1ML |
| Bacterial and virus strains | ||
| HCMV AD169-GFP | Dong Yu | N/A |
| HCMV TB40/E | Domenico Tortorella (Mount Sinai, New York, NY, USA) | N/A |
| HCMV TB40/E-GFP | Eain Murphy (SUNY, Syracuse, NY, USA) | N/A |
| HSV-1-GFP-Us11 | Dr. Ian Mohr (NYU Schiool of Medicine, New York, NY, USA) | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| DMEM | Corning | cat# 10-013-CV |
| DMEM without L-Glutamine/L-Methionine/L-Cystine | Corning | cat# MT17204CI |
| 25mM HEPES | Corning | cat# 25060CI |
| 100 U/mL penicillin-100μg/mL streptomycin | Corning | cat# MT-30-002-Cl |
| pp242 | Invivogen | cat# inh-pp242 |
| RNAimax | Invitrogen | cat# 13778075 |
| Opti-MEM | Gibco | cat# 31985070 |
| TRIzol | Invitrogen | cat# 15596018 |
| GlycoBlue | Invitrogen | cat# AM9515 |
| nuclease-free water | Invitrogen | cat# AM9937 |
| qScript XLT cDNA SuperMix | QuantaBio | cat# 95161-500 |
| SsoAdvanced Universal SYBR Green Supermix | Bio-Rad | cat# 1725275 |
| Phosphonoacetic acid | Sigma | cat# P6909 |
| EasyTag Express 35S Labeling Mix | Perkin Elmer | cat# NEG772007MC |
| CytoScint ES scintillation fluid | MP Biomedicals, Inc | cat# 0188245301 |
| Cycloheximide | Sigma | cat# C7698 |
| RiboLock RNase Inhibitor | ThermoFisher Scientific | cat# EO0381 |
| cOmplete mini EDTA-free Protease Inhibitor Cocktail tablet | Roche | cat# 05892791001 |
| Critical commercial assays | ||
| Allprep DNA Kit | Qiagen | cat# 80204 |
| RNA Clean and Concentrator-5 kit | Zymo Research | cat# R1014 |
| NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina | New England Biolabs | cat# E7760S |
| Deposited data | ||
| RNA sequencing datasets | European Nucleotide Archive (ENA) | BioProject ID PRJEB45749 |
| Experimental models: Cell lines | ||
| Normal human dermal fibroblasts (NHDF) | Lonza | cat# CC-2509 |
| African green monkey cells (Vero) | ATCC | cat# CCL-81 |
| Human retinal pigment epithelial cells (ARPE-19) | ATCC | cat# CRL-2302 |
| Oligonucleotides | ||
| DNA oligonucleotides for PCR | This paper and other sources | Table S5 |
| siRNAs | Qiagen, Sigma | Table S5 |
| Software and algorithms | ||
| GraphPad Prism (Version 9) | Graphpad | RRID: SCR_002798 |
| RIVET | Ernlund et al., 2018 | N/A |
| Kallisto | Bray et al., 2016 | N/A |
| ComBat-seq | Zhang et al., 2020 | N/A |
| Rstudio using ggplot2 | Wickham, 2016 | N/A |
| REACTOME | Jassal et al., 2020 | N/A |
| TrimGalore | https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/ | N/A |
| STAR | Dobin et al., 2013 | N/A |
| SAMtools | Li et al., 2009 | N/A |
| BEDtools | Quinlan, 2014 | N/A |
| biomaRt | Durinck et al., 2005; Durinck et al., 2009 | N/A |
| Other | ||
| Nitrocellulose membranes | GE Healthcare | cat# 10600002 |
| Whatman Grade GF/C Glass Microfiber Filters | GE Healthcare | cat# 18822-025 |
| thinwall polypropylene ultracentrifuge tube | Beckman Coulter | cat# 331372 |
| SW41Ti rotor | Beckman Coulter | cat# 331362 |
| density gradient fractionation system | Brandel | cat# BR-188 |