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. 2022 May 10;13:829406. doi: 10.3389/fimmu.2022.829406

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Copyright © 2022 Lellouch, Andrews, Saviane, Ng, Schol, Goutard, Gama, Rosales, Colvin, Lantieri, Randolph, Benichou and Cetrulo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Clinical assessment of successful VCA tolerance. Images of tolerant VCAs from R3 (A), R4 (C), and R5 (E) taken on POD 251, 387, and 250, respectively, compared to a representative rejected VCA from untreated control R6 on POD 11 (G). H&E staining of VCA biopsies from R3 (B), R4 (D), and R5 (F), taken on POD 151, 387, and 250 respectively, demonstrating absence of rejection (Banff Grade 0). Whereas H&E staining of VCA skin from class I mismatch untreated control R6 shows focal epidermal necrosis and capillary thrombosis (Banff Grade IV) on POD 11 (H). (POD, post-operative day; VCA, vascularized composite allograft).