Table 2.
High-confidence tick proteins with reported functions in the hemolymph of H. flava ticks
| No. | Protein | Alignment | iBAQ (× 106) | ||||
|---|---|---|---|---|---|---|---|
| ID | Length (amino acids) | Entry and overview | E value | Score | Identity (%) | ||
| I. Enzymes | |||||||
| 1 | Cl-k.18156 ① | 376 | L7M876, tick serine protease, Rhipicephalus pulchellus | 0 | 1897 | 84.6 | 5.96 ± 1.32 |
| 2 | Cl-k.17502 ① | 484 | A0A131Z7A0, tick serine protease, Rhipicephalus appendiculatus | 0 | 1451 | 67.8 | 0.39 ± 0.04 |
| 3 | Cl-k.19217 ① | 437 | A0A1E1X8K7, serine protease, Amblyomma aureolatum | 0 | 1907 | 85.1 | 2.13 ± 0.47 |
| 4 | Cl-k.19341 | 348 | A1IHG0, longipain, H. longicornis | 0 | 1814 | 93.5 | 7.34 ± 1.74 |
| 5 | Cl-k.18108 | 443 | A0A097CK68, enolase, H. flava | 0 | 2233 | 99.5 | 1.16 ± 0.35 |
| 6 | Cl-k.14381 | 507 | A0A6M2D6D9, serine carboxypeptidase, R. microplus | 0 | 2115 | 83.6 | 0.49 ± 0.15 |
| 7 | Cl-k.18093 | 164 | A0A131XJQ8, metalloproteinase, Hyalomma excavatum | 1.7E−91 | 686 | 75.0 | (7) 221.26 ± 58.79 |
| 8 | Cl-k.18993 | 398 | Q2WFX6, aspartic protease, H. longicornis | 0 | 1990 | 95.9 | 1.47 ± 0.34 |
| 9 | Cl-k.7217 | 397 | A0A034WWI5, heme-binding aspartic peptidase, R. microplus | 0 | 1343 | 68.0 | 3.03 ± 0.93 |
| 10 | Cl-k.14313 | 561 | A0A1E1XAU4, cysteine proteinase, A. aureolatum | 0 | 2602 | 84.4 | 5.24 ± 1.17 |
| 11 | Cl-k.18480 | 326 | A0A023FWK4, cathepsin L, Amblyomma parvum | 0 | 1604 | 88.7 | 99.93 ± 26.58 |
| 12 | Cl-k.24797 | 110 | A0A023GJU1, cathepsin C, Amblyomma triste | 2.6E−70 | 563 | 92.7 | 2.08 ± 0.83 |
| 13 | Cl-k.18626 | 416 | L7M0J1, phospholipase a2, R. pulchellus | 0 | 1704 | 80.1 | 25.19 ± 5.41 |
| 14 | Cl-k.30316 ② | 566 | Q9U6M8, carboxylic ester hydrolase, R. microplus | 5.1E−171 | 1290 | 47.3 | 10.59 ± 2.67 |
| 15 | Cl-k.24717 ② | 564 | A0A6M2CHI4, carboxylic ester hydrolase, R. microplus | 0 | 1366 | 49.7 | 0.25 ± 0.05 |
| 16 | Cl-k.18635 | 521 | A5LHV9, disulfide isomerase, H. longicornis | 0 | 2514 | 96.2 | 1.74 ± 0.43 |
| 17 | Cl-k.17461 | 163 | F2Z7L0, lysozyme, H. longicornis | 3.1E−70 | 544 | 87.1 | 2.44 ± 0.86 |
| 18 | Cl-k.17638 | 397 | G8C7A0, lysosomal acid phosphatase, H. longicornis | 0 | 1878 | 93.5 | 75.50 ± 18.32 |
| 19 | Cl-k.18136 | 233 | Q6JVN0, glutathione S-transferase, H. longicornis | 3.5E−159 | 1144 | 93.7 | 1.74 ± 0.50 |
| 20 | Cl-k.18835 | 526 | A0A131YK94, superoxide dismutase (Cu–Zn), R. appendiculatus | 0 | 1475 | 61.8 | 18.83 ± 4.63 |
| 21 | Cl-k.19315 ③ | 316 | A0A131YMH9, chitinase, R. appendiculatus | 0 | 1777 | 85.6 | 1.46 ± 0.44 |
| 22 | Cl-k.18448 ③ | 536 | A0A023FND8, chitinase, A. cajennense | 0 | 1919 | 80.3 | 21.40 ± 4.31 |
| 23 | Cl-k.17863 | 367 | A0A286R6W4, fructose-bisphosphate aldolase, Dermacentor silvarum | 0 | 1796 | 94.2 | 2.26 ± 0.57 |
| 24 | Cl-k.35642 | 331 | A0A1E1X9Y4, alpha-L-fucosidase, A. aureolatum | 0 | 1414 | 81.7 | 1.87 ± 0.38 |
| 25 | Cl-k.18180 | 430 | A0A2P1DPZ4, glyceraldehyde-3-phosphate dehydrogenase, H. flava | 0 | 1730 | 100 | 2.37 ± 0.62 |
| 26 | Cl-k.17933 | 549 | A0A6M2CTD4, ATP synthase subunit beta, R. microplus | 0 | 2628 | 96.3 | 0.39 ± 0.02 |
| II. Enzyme inhibitors | |||||||
| 27 | Cl-k.20245 ④ | 256 | A0A5P8H6S1, serpin-a, H. longicornis | 9.3E−97 | 753 | 61.9 | 55.88 ± 13.09 |
| 28 | Cl-k.17714 ④ | 415 | A0A5P8H6S1, serpin-a, H. longicornis | 6.9E−169 | 1246 | 63.4 | 0.25 ± 0.24 |
| 29 | Cl-k.16905 ④ | 143 | A0A5P8H6S1, serpin-a, H. longicornis | 6.4E−49 | 422 | 61.8 | 32.01 ± 6.67 |
| 30 | Cl-k.18212 ④ | 427 | A0A5P8H6S1, serpin-a, H. longicornis | 0 | 1727 | 87.5 | 45.04 ± 10.59 |
| 31 | Cl-k.19946 ④ | 406 | Q75Q63, serpin-2 , H. longicornis | 0 | 1686 | 82.4 | 11.48 ± 2.66 |
| 32 | Cl-k.16646 ④ | 201 | A0A6M2E637, tick serpins 8, A. tuberculatum | 7.5E−68 | 555 | 63.0 | ⑻ 178.59 ± 44.58 |
| 33 | Cl-k.22217 ④ | 398 | A0A023GN51, tick serpins 13, A. triste | 9.5E−165 | 1216 | 61.8 | 38.49 ± 10.90 |
| 34 | Cl-k.14429 | 421 | A0A023G8Z1, serine proteinase inhibitor, A. triste | 0 | 1409 | 65.5 | 71.47 ± 17.26 |
| 35 | Cl-k.18644 ⑤ | 229 | A0A224YJB7, α2-macroglobulin splicing variant, Rhipicephalus zambeziensis | 3.7E−141 | 1119 | 91.7 | ⑸ 435.08 ± 100.49 |
| 36 | Cl-k.18677 ⑤ | 1142 | A0A1E1XEL3, α-macroglobulin, A. aureolatum | 0 | 5401 | 89.6 | ⑹ 243.02 ± 53.71 |
| 37 | Cl-k.19779 ⑤ | 1820 | A0A1E1XL07, α-macroglobulin, A. sculptum | 0 | 6650 | 76.1 | 1.10 ± 0.27 |
| 38 | Cl-k.18726 et al. | 1915 | A0A023FNM2, α2-macroglobulin splicing variant, Amblyomma cajennense | 0 | 5819 | 78.9 | 35.72 ± 8.99 |
| 39 | Cl-k.18944 | 269 | A0A023GP16, Kazal-type serine protease inhibitor, A. triste | 5.8E−155 | 1123 | 84.0 | 8.02 ± 1.79 |
| 40 | Cl-k.12087 ⑥ | 183 | A0A6B9DA14, cystatin, H. flava | 0 | 757 | 100 | 15.11 ± 4.89 |
| 41 | Cl-k.17388 ⑥ | 185 | A0A3G6VF56, cystatin, H. flava | 1.9E−100 | 745 | 99.3 | 65.43 ± 15.54 |
| 42 | Cl-k.20981 ⑥ | 164 | A0A6M3YRY3, cystatin, H. flava | 4.2E−89 | 667 | 100 | 15.73 ± 4.93 |
| 43 | Cl-k.21288 | 228 | A0A023GEH6, thyropin, A. triste | 1.5E−94 | 723 | 60.0 | 22.19 ± 7.67 |
| 44 | Cl-k.23450 | 229 | A0A023GAB0, thyropin, A. triste | 2.2E−89 | 689 | 59.4 | 35.86 ± 8.77 |
| III. Immune-related proteins | |||||||
| 45 | Cl-k.18200 ⑦ | 84 | A0A6G5A751, microplusin, R. microplus | 9.5E−31 | 260 | 54.8 | ⑴ 6514.56 ± 803.43 |
| 46 | Cl-k.18906 ⑦ | 134 | A0A6G5A751, microplusin, R. microplus | 8.0E−34 | 295 | 52.5 | ⑷ 699.92 ± 23.27 |
| 47 | Cl-k.20235 ⑦ | 87 | A0A6G5A751, microplusin, R. microplus | 2.1E−29 | 266 | 46.2 | 61.60 ± 17.16 |
| 48 | Cl-k.3924 | 78 | A0A2D1CLH7, defensin DFS2, H. longicornis | 2.1E−48 | 361 | 83.6 | 11.98 ± 3.53 |
| 49 | Cl-k.23590 ⑧ | 1612 | A0A131ZDX3, TIL domain-containing protein, R. appendiculatus | 0 | 7053 | 77.0 | 11.48 ± 2.56 |
| 50 | Cl-k.13586 ⑧ | 2610 | A0A131Z678, TIL domain-containing protein, R. appendiculatus | 0 | 8535 | 77.2 | 9.72 ± 2.21 |
| 51 | Cl-k.18775 ⑧ | 2252 | A0A131YJS1, TIL domain-containing protein, R. appendiculatus | 0 | 8966 | 68.9 | 1.42 ± 0.30 |
| 52 | Cl-k.25067 ⑧ | 109 | A0A6M2CNI0,TIL domain-containing protein, R. microplus | 4.1E−39 | 329 | 69.9 | 26.91 ± 4.60 |
| 53 | Cl-k.18521 ⑨ | 235 | F0J8I6, ixoderin, A. variegatum | 5.4E−93 | 707 | 66.3 | 6.23 ± 1.40 |
| 54 | Cl-k.17959 ⑨ | 313 | A0A1E1XEF5, ixoderin, A. aureolatum | 5.7E−153 | 1118 | 73.8 | 6.92 ± 1.81 |
| 55 | Cl-k.19166 | 177 | A0A6M2D1K3, C2b, R. microplus | 6.9E−55 | 488 | 62.3 | 2.75 ± 0.88 |
| 56 | Cl-k.21838 | 428 | A0A7L9DI94, C3, Ixodes ricinus | 1.0E−134 | 1104 | 50.9 | 10.48 ± 2.55 |
| 57 | Cl-k.14141 | 152 | A0A224Z7V2, serum amyloid A protein, R. zambeziensis | 1.4E−77 | 592 | 72.7 | 28.19 ± 6.81 |
| 58 | Cl-k.17842 | 332 | A0A0S3Q1T5, leucine-rich repeat containing protein, H. longicornis | 0 | 1464 | 90.1 | 19.96 ± 4.17 |
| 59 | Cl-k.18342 | 233 | A0A0M3TC17, AV422, H. flava | 8.4E−171 | 1221 | 100 | 12.15 ± 2.84 |
| 60 | Cl-k.18520 ⑩ | 181 | Q08G07, Hq05, Haemaphysalis qinghaiensis | 1.1E−129 | 940 | 97.2 | 6.53 ± 1.43 |
| 61 | Cl-k.18575 ⑩ | 180 | G3BJU6, immunogenic protein, H. longicornis | 4.5E−120 | 877 | 93.9 | 16.62 ± 4.03 |
| IV. Transporters | |||||||
| 62 | Cl-k.25224 ⑪ | 1538 | Q5EG54, Vg, D. variabilis | 0 | 5360 | 67.5 | 0.21 ± 0.10 |
| 63 | Cl-k.16576 ⑪ | 686 | E1CAX9, vitellogenin-1, H. longicornis | 0 | 1248 | 98.5 | 126.50 ± 31.13 |
| 64 | Cl-k.19213-k18886 ⑪ | 351 | B1B544, vitellogenin-2, H. longicornis | 8.7E−86 | 732 | 98.3 | ⑶ 1606.10 ± 295.28 |
| 65 | Cl-k.18067 ⑪ | 463 | B1B544, vitellogenin-2, H. longicornis | 0 | 2072 | 85.1 | ⑵ 1829.13 ± 312.65 |
| 66 | Cl-k.19483 ⑪ | 1386 | B1B544, vitellogenin-2, H. longicornis | 0 | 4657 | 62.4 | ⑽ 138.31 ± 29.98 |
| 67 | Cl-k.16789 ⑪ | 114 | E1CAY0, vitellogenin-3, H. longicornis | 3.1E−62 | 535 | 85.7 | 95.40 ± 25.38 |
| 68 | Cl-k.18851-18114 ⑪ | 2279 | G9M4L6, vitellogenin B, H. longicornis | 0 | 13,028 | 85.9 | 63.60 ± 12.52 |
| 69 | Cl-k.21299 | 207 | M5AYG7, ferritin 2, H. longicornis | 9.9E−133 | 965 | 93.5 | 22.51 ± 4.62 |
| 70 | Cl-k.19103 | 152 | A0A023G718, fatty acid-binding protein, A. triste | 4.6E−83 | 628 | 85.4 | 15.26 ± 2.76 |
| V. Muscle proteins | |||||||
| 71 | Cl-k.18720 | 877 | J7LVN2, paramyosin, H. longicornis | 0 | 4196 | 98.2 | 0.53 ± 0.13 |
| 72 | Cl-k.18460 | 424 | A8E4J9, calreticulin, H. qinghaiensis | 0 | 2247 | 99.5 | 2.42 ± 0.76 |
| 73 | Cl-k.18394 | 52 | A0A131ZAE8, tropomyosin, R. appendiculatus | 0 | 1423 | 93.2 | 3.76 ± 1.07 |
| 74 | Cl-k.18452 | 83 | A0A0N6X2B1, muscle LIM protein, H. longicornis | 7.8E−57 | 417 | 95.8 | 4.98 ± 1.14 |
| VI. Heat shock proteins | |||||||
| 75 | Cl-k.18505 ⑫ | 655 | A0A097A1J8, heat shock 70 kDa protein 8, H. flava | 0 | 3290 | 100 | 2.09 ± 0.55 |
| 76 | Cl-k.18161 ⑫ | 683 | E4W3Z2, heat shock 70 kDa protein 5, H. longicornis | 0 | 3317 | 99.1 | 0.45 ± 0.22 |
| Other | |||||||
| Cl-k.18334 | 255 | A0A023FPM9, glycine-rich secreted cement protein, A. cajennense | 1.1E−118 | 884 | 74.0 | ⑼ 170.44 ± 38.51 | |
Proteins sharing the same number in the Protein ID column belong to the same family. Length indicates the number of amino acid residues of protein fractions detected by MS. The number in the iBAQ column indicates the top 10 most abundant peptides detected by MS