FIGURE 3.

Cancer‐associated fibroblast (CAF)‐derived exosomal miR‐106a‐5p accelerated the progression of nasopharyngeal carcinoma (NPC). Nasopharyngeal carcinoma–derived CAFs were transfected with mimics NC, miR‐106a‐5p mimics, inhibitor NC, or miR‐106a‐5p inhibitor. Exosomes were isolated and cocultured with HONE1 or SUNE‐1 cells. A, RT‐qPCR analysis of miR‐106a‐5p (n = 3). B, Cell proliferation analysis with CCK‐8 (n = 3). C, Colony formation of HONE1 and SUNE‐1 cells (magnification, 1×; n = 3). D, The migration of HONE1 and SUNE‐1 cells was assessed with wound‐healing assays (magnification, 20×; scale bar, 500 µm; n = 3). E, The invasion of HONE1 and SUNE‐1 cells was evaluated by transwell assays (magnification, 50×; scale bar, 200 µm; n = 3). F, HONE1 and SUNE‐1 cells were subcutaneously inoculated into nude mice. Excised tumors were imaged, measured, and weighed (n = 4). G, HONE1 and SUNE‐1 cells were intravenously injected into nude mice. Lungs were excised and imaged. Metastatic nodules were calculated, and H&E staining was performed for evaluating lung damage (magnification, 200×; scale bar, 50 µm; n = 4). H, Protein levels of FBXW7, TRIM24, and SRGN. *p < 0.05, **p < 0.01, and ***p < 0.001