FIGURE 4.

Exosomal miR‐106a‐5p reversed TRIM24 knockdown–mediated suppression of nasopharyngeal carcinoma (NPC) progression. Sh‐NC– or sh‐TRIM24–transfected HONE1 and SUNE‐1 cells were coincubated with miR‐106a‐5p–overexpressing cancer‐associated fibroblast (CAF)‐derived exosomes. A, Protein levels of TRIM24. B, Cell proliferation analysis (n = 3). C, Colony formation of HONE1 and SUNE‐1 cells (magnification, 1×; n = 3). D, The migration of HONE1 and SUNE‐1 cells was assessed with wound‐healing assays (magnification, 20×; scale bar, 500 µm; n = 3). E, The invasion of HONE1 and SUNE‐1 cells was evaluated by transwell assays (magnification, 50×; scale bar, 200 µm; n = 3). F, Excised tumors were imaged, measured, and weighed (n = 4). G, HONE1 and SUNE‐1 cells were intravenously injected into nude mice. Lungs were excised and imaged. Metastatic nodules were calculated, and H&E staining was performed for evaluating lung damage (magnification, 200×; scale bar, 50 µm; n = 4). H, Protein levels of TRIM24 and SRGN. *p < 0.05, **p < 0.01, and ***p < 0.001