FIGURE 3.

The adhesive activity of CADM1 is essential for promoting liver infiltration of T‐cell lymphoma cells. (A) Generation of Jurkat cells stably expressing CADM1 or CADM1^F86S. (B) Adhesion assay on recombinant CADM1‐binding proteins. Jurkat cells with vector, CADM1, and CADM1^F86S were plated onto coverslips coated with the indicated proteins, and the number of attached cells was counted. Means ± SD of relative cell number in three experiments are shown. *p < 0.05 using one‐sample t test (versus IgG). (C) Cell aggregation activity of CADM1 and CADM1^F86S was examined by incubating for 24 h after plating 1 × 10⁴ single cells in a 24‐well plate. Bars, 50 µm. (D) Representative photographs of the livers after the tail vein injection of EL4 cells expressing CADM1. Bar, 1 cm. (E) The number of tumor nodules on the liver surface. Means ± SD and each value of six experiments are shown. *p < 0.05; **p < 0.01 using Mann–Whitney U‐test. (F) Subcutaneous tumor formation of EL4/vector and EL4/CADM1 cells. Means ± SD of tumor volume in five mice are shown