TABLE 1.
Primer sequences for RT‐qPCR
| Gene | Primer sequence (5′‐3′) |
|---|---|
| USP10 | F: GAGGTGGAAAGTTGTTTGCCTGG |
| R: TGCTCCATCTCTAAGAGGTGGC | |
| Cyr61 | F: GGAAAAGGCAGCTCACTGAAGC |
| R: GGAGATACCAGTTCCACAGGTC | |
| IL‐13 | F: CAGCCCTCAGCCATGAAATA |
| R: CTTGAGTGTGTAACAGGCCATTCT | |
| IL‐6 | F: GATAAGCTGGAGTCACAGAAGG |
| R: TTGCCGAGTAGATCTCAAAGTG | |
| IL‐1β | F: CCACAGACCTTCCAGGAGAATG |
| R: GTGCAGTTCAGTGATCGTACAGG | |
| iNOS | F: GCTCTACACCTCCAATGTGACC |
| R: CTGCCGAGATTTGAGCCTCATG | |
| Arg1 | F: TCATCTGGGTGGATGCTCACAC |
| R: GAGAATCCTGGCACATCGGGAA | |
| ZO‐1 | F: GTCCAGAATCTCGGAAAAGTGCC |
| R: CTTTCAGCGCACCATACCAACC | |
| E‐cadherin | F: GCCTCCTGAAAAGAGAGTGGAAG |
| R: TGGCAGTGTCTCTCCAAATCCG | |
| Vimentin | F: AGGCAAAGCAGGAGTCCACTGA |
| R: ATCTGGCGTTCCAGGGACTCAT | |
| N‐cadherin | F: CCTCCAGAGTTTACTGCCATGAC |
| R: GTAGGATCTCCGCCACTGATTC | |
| Galectin | F: ACACCCAGATCGACAACTCCTG |
| R: CAAACAGGTGCTGACCATCCAC | |
| GAPDH | F: CGGAGTCAACGGATTTGGTCGTAT |
| R: AGCCTTCTCCATCATTGTGGTGAAGAC |
Arg1, arginase 1; Cyr61, cysteine rich angiogenic inducer 61; F, forward; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; IL, interleukin; iNOS, nitric oxide synthase 2; R, reverse; RT‐qPCR, reverse transcription quantitative polymerase chain reaction; USP10, ubiquitin specific peptidase 10; ZO‐1, tight junction protein 1.