TABLE 5.
Bacterial strains and vectors used in the study
| Strain or vector | Description | Source or reference |
|---|---|---|
| E. coli strains and genotypes | ||
| DH5α | F− ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) phoA supE44 λ− thi-1 gyrA96 relA1; host for subcloning of genes and vectors | Vazyme Co., Ltd. |
| ATCC 35218 | Beta lactamase producer; does not contain the stx1 or stx2 genes for Shiga toxin production | ATCC |
| ΔwaaC | ATCC 35218 with deletion of waaC | This work |
| ΔwaaY | ATCC 35218 with deletion of waaY | This work |
| ΔlpxA | ATCC 35218 with deletion of lpxA | This work |
| ΔwaaC/waaC | E. coli ΔwaaC with pUC57-waaC | This work |
| ΔwaaY/waaY | E. coli ΔwaaY with pUC57-waaY | This work |
| ΔlpxA/lpxA | E. coli ΔlpxA with pUC57-lpxA | This work |
| Vectors | ||
| pUC57 | Expression vector of E. coli | Thermo Scientific |
| pUC57-mcr1 | pUC57 with phosphoethanolamine-lipid A transferase mcr-1 (gene ID 39727008) | This work |
| pUC57-waaC | pUC57 with waaC | This work |
| pUC57-waaY | pUC57 with waaY | This work |
| pUC57-lpxA | pUC57 with lpxA | This work |
| pTargetF | sgRNA vector | 51 |
| pCas | Gene knockout vector with Cas9 | 51 |
| pTargetF-waaC | Expresses sgRNA-waaC | This work |
| pTargetF-waaY | Expresses sgRNA-waaY | This work |
| pTargetF-lpxA | Expresses sgRNA-lpxA | This work |