TABLE 7.
Primers used to knock out lpxL and waaC by the CRISPR system
| Primer | Sequencea | Description |
|---|---|---|
| lpxA-N20-F | TCCTAGGTATAATACTAGTGTGATGGTTGGCGGCTGCTCGTTTTAGAGCTAGAAATAGC | sgRNA of lpxA |
| waaC-N20-F | TCCTAGGTATAATACTAGTCCCAGGGATTAAGTTTGACTGTTTTAGAGCTAGAAATAGC | sgRNA of waaC |
| waaC-N20-F | TCCTAGGTATAATACTAGTACCCTTCATTTCGTACTTTTGTTTTAGAGCTAGAAATAGC | sgRNA of waaY |
| sgRNA-R | ACTAGTATTATACCTAGGACTGAGCTAGCT | Amplified plasmid pTargetF |
| lpxA-ssDNA | CGCAGTCCATCAGTTCTGCATCATTGGTGCGCACGTGATGGGCGCAGGACGTCCCTCCTTATGTCATTGCGCAGGGTAAC | Template for homologous recombination |
| waaC-ssDNA | CGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGC | Template for homologous recombination |
| waaY-ssDNA | CCAATAAATAAAAGTCATTGAGTGTATTTAACCCTTCATTTCGTACTGGGTTTGCTCAAAAAGGCGTTCGTAATAATCACCTTT | Template for homologous recombination |
a
Underlining indicates 20 bp of single guide RNA, which paired with complementary bases of target gene.