FIGURE 3.

Kidney injury and oxidative stress markers during NMP. Porcine kidneys were perfused for 4 h with blood (control) with Aqix supplemented with a colloid (2.2% BSA or 3.5% Dex) or with a colloid (BSA or Dex) and RBC. A, ASAT and LDH levels during 4 h NMP, *P < 0.001 Aqix + BSA vs all other experimental groups. B, uNAG levels during 4 h NMP. C, Histology damage scores from sections taken after 4 h NMP. Negative control samples are additional kidney tissue samples taken directly after death of the pig, **P < 0.05. D, Total TBARS production during 4 h perfusion, **P < 0.04. Every group contains 6 kidneys. Data are presented as mean ± SEM, except in Figure 3C, data are presented as median ± interquartile range. ASAT, aspartate aminotransferase; BSA, bovine serum albumin; Dex, dextran 40; LDH, lactate dehydrogenase; NMP, normothermic machine perfusion; RBC, red blood cell; TBARS, thiobarbituric acid-reactive substances; uNAG, urinary N-acetyl-beta-d-glucosaminidase.