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. 2022 May 24;12:8763. doi: 10.1038/s41598-022-12829-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Genetic analysis of iron uptake genes of AB5075 (A) and AMA40 (B) A. baumannii strains. qRT-PCR of genes associated with iron uptake, pirA, piuA, bauA and bfnH expressed in cation adjusted Mueller Hinton or cation adjusted Mueller Hinton supplemented with 50% HU. Fold changes were calculated using double ΔCt analysis. At least three independent samples were used, and four technical replicates were performed from each sample. The cation adjusted Mueller Hinton was used as reference. Statistical significance (p < 0.05) was determined by ANOVA followed by Tukey’s multiple-comparison test, one asterisks: p < 0.05; two asterisks: p < 0.01 and three asterisks: p < 0.001.