Fig. 1.

Guanine deaminase (GDA) expression levels were increased with uric acid and p21 in seborrhoeic keratosis (SK). (a) Real-time PCR to determine the relative ratios of GDA mRNA in lesional skin (L) samples compared with non-lesional skin (N) samples obtained from 10 patients with seborrhoeic keratosis (SK). (b-d) Representative immunofluorescent staining using: (b) anti-GDA, (c) anti-uric acid, or (d) anti-p21 antibody, in lesional skin samples compared with that of non-lesional skin samples from 4 other patients with SK with increased GDA mRNA levels. Nuclei were counterstained with Hoechst 33258 (bar 0.05 mm). Intensities of immunofluorescence staining were measured using Wright Cell Imaging Facility (WCIF) ImageJ software. *p < 0.05 vs. control keratinocytes.