Table II.

Relative skin recovery depending on perfusate composition^a

Biomarker	Perfusate compositionb	Relative skin recovery (%)		p-valuec	n (skin donor identifier)d
		Mean ± SD	CV
CCL27/CTACK	Standard	n/a	n/a	n/a	1 (D41)
CCL27/CTACK	Standard	n/a	n/a	n/a	1 (D41)
	+ lactic acid	n/a	n/a
CXCL1/GROα	Standard	7.9 ± 1.0	12.5	0.6348	1 (D31)
	+ lactic acid	7.4 ± 1.1	14.8
CXCL7/NAP-2	Standard	5.7 ± 0.4	7.9	0.0889	1 (D26)
	+ lactic acid	9.7 ± 2.3	23.5
CXCL10/IP-10	Standard	0.6 ± 0.2	29.2	0.2647	1 (D32)
	+ lactic acid	0.9 ± 0.3	30.6
EGF	Standard	12.7 ± 0.5	4.2	0.0044	1 (D23)
	+ lactic acid	9.8 ± 0.2	1.7
GM-CSF	Standard	13.3 ± 2.0	15.1	0.4953	1 (D31)
	+ lactic acid	12.4 ± 0.5	3.7
IFN-γ	Standard	0.0 ± n/a	n/a	n/a	1 (D11)
	+ lactic acid	0.0 ± n/a	n/a
IL-1α	Standard	14.6 ± 2.5	16.8	0.2219	1 (D5)
	+ lactic acid	12.2 ± 0.9	7.5
IL-6	Standard	8.9 ± 2.3	25.8	0.0699	2 (D17/D26)
	+ lactic acid	11.4 ± 1.9	16.4
IL-8	Standard	18.4 ± 1.4	7.7	0.2912	1 (D30)
	+ lactic acid	16.7 ± 2.0	11.9
IL-17	Standard	11.2 ± 0.6	5.1	0.0693	1 (D25)
	+ lactic acid	8.7 ± 1.3	15.1
IL-22	Standard	7.7 ± 0.8	10.4	0.1030	1 (D11)
	+ lactic acid	11.4 ± 2.3	20.4
IL-23	Standard	5.2 ± 0.8	14.4	0.8047	1 (D23)
	+ lactic acid	4.8 ± 2.6	54.3
MIF	Standard	n/a	n/a	n/a	1 (D1)
	+ lactic acid	n/a	n/a
TNF-α	Standard	7.5 ± 1.9	25.4	0.0555	2 (D17/D25)
	+ lactic acid	10.1 ± 2.1	20.5
TSLP	Standard	4.0 ± 0.9	23.7	0.6458	1 (D23)
	+ lactic acid	4.3 ± 0.7	16.8
VEGF	Standard	4.8 ± 1.1	22.3	0.2679	1 (D32)
	+ lactic acid	3.8 ± 0.7	19.1

^aData are depicted in Fig. S41.

^bRelative skin recoveries obtained in response to perfusion with standard perfusate or perfusate supplemented with lactic acid to a final concentration of 4 mM.

^cp-values are obtained using t-tests with Welch’s correction. n/a=not applicable.

^dThe perfusate-dependent relative skin recovery of each biomarker was investigated in skin from 1 donor, except for IL-6 and TNF-α, which were assessed in skin from 2 donors. Significant value is given in bold.

SD: standard deviation; CV: coefficient of variation.