Fig. 3.

The cell proliferation and metabolic readiness of normal human epidermal keratinocytes (NHEKs) in 3 conditions (Untreated NHEKs, P-DMSC-treated NHEKs and C-DMSC-treated NHEKs). (A) The OD (450 nm) of NHEKs by a CCK8 assay based on 1×10⁴ cells (t-test, data are expressed as mean with standard deviation (SD)). (B) The oxygen-consumption rate (OCR) based on 1×10⁶ NHEKs detected at different time points in three conditions (Data are expressed as mean with SD). OCR under oligomycin, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP), and antimycin A/rotenone treatments (Data are expressed as Mean with SEM). (C) The extracellular acidification rates (ECAR) based on 1×10⁶ NHEKs detected at different time points in 3 conditions (Data are expressed as mean with SD). ECAR under glucose, oligomycin, and 2-DG.