Fig 5. Effect of LED light exposure on the morphology and survival of RPE Cells.

(A) The RPE cells became more spindle-like in shape, and some detached from the culture plate after 16 h of light exposure. This was more evident in the blue LED irradiated cells. Scale bar represents 50 μm. (B) Blue light irradiation significantly induced more cell death compared to white light and the dark control, as shown by trypan blue and MTT assays. Cell viability dropped to 86% and 59% in white and blue LED light, respectively, under MTT assay. (n = 3, one way ANOVA, * p<0.05, ** p<0.01.) The values are expressed as Mean ± SEM.