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. 2022 May 24;17(5):e0266015. doi: 10.1371/journal.pone.0266015

Table 1. Oligonucleotides used for quantitative real-time PCR.

Target Sequence
3CLpro / m3CLpro Forward primer: TTGGGTCGCAGTTCTTGTTTG
Reverse primer: TGCCTTGACATTCTCGATGGT
Probe: VIC-TCGCTGTGATCGTCACTTGACAATG-BHQ2
Ubiquitin C (UBC) Forward primer: CATGCTGGCACAGACTTAGAAG
Reverse primer: GCAGCGTACAACCAAGCTAAAC
Probe: VIC-ACAGGCAAACAGCACAAGCAGC-BHQ2

All oligonucleotides were synthesized by DNK-sintez (Russia). VIC– 2′-chloro-7′phenyl-1,4-dichloro-6-carboxy-fluorescein; ROX—carboxyrhodamine; BHQ2 –Black Hole Quencher 2.