Table 1. Oligonucleotides used for quantitative real-time PCR.
Target | Sequence | |
---|---|---|
3CLpro / m3CLpro | Forward primer: | TTGGGTCGCAGTTCTTGTTTG |
Reverse primer: | TGCCTTGACATTCTCGATGGT | |
Probe: | VIC-TCGCTGTGATCGTCACTTGACAATG-BHQ2 | |
Ubiquitin C (UBC) | Forward primer: | CATGCTGGCACAGACTTAGAAG |
Reverse primer: | GCAGCGTACAACCAAGCTAAAC | |
Probe: | VIC-ACAGGCAAACAGCACAAGCAGC-BHQ2 |
All oligonucleotides were synthesized by DNK-sintez (Russia). VIC– 2′-chloro-7′phenyl-1,4-dichloro-6-carboxy-fluorescein; ROX—carboxyrhodamine; BHQ2 –Black Hole Quencher 2.