Protein structure and expression analysis of SlIMP3. (a) Protein sequences alignment and conserved domain analysis of SlIMP1, SlIMP2, and SlIMP3. (b) Phylogenetic tree of IMPs proteins in Arabidopsis thaliana (At), Nicotiana tabacum (Nt), and Solanum lycopersicum (Sl) constructed by the neighbour‐joining method. (c) Differences in relative expression level of SlIMPs in various tomato tissues: R root, S shoot, L leaf, F flower, IMG immature green fruit, MG mature green fruit, BF break fruit, OF orange fruit, RF red fruit. All data contain three replicates, and the standard deviation (SD) is given by error bar. Statistical significance between SlIMP3 and SlIMP1/SlIMP2 was indicated by asterisk (***P < 0.001). (d) GUS staining of ProSlIMP3::GUS transgenic plants in different tissues: S shoot, L leaf, F flower, IMG immature green fruit, MG mature green fruit, OF orange fruit. Scale bars, 1 mm.