Overexpression of SlIMP3 delays fruit softening and increased cell wall thickness in ‘Micro‐tom’ tomato. (a) Red fruits of WT, OESlIMP3, and ASSlIMP3 lines were stored at 25°C for 0 and 26 days. Scale bars, 5 mm. (b) Water loss rate of fruits in WT, OESlIMP3, and ASSlIMP3 lines. Statistics of water loss rate for every two days. The data contain four replicates: the error bar represents the SD. (c) Firmness of fruits at different developmental stages: GF green fruit, OF orange fruit, RF red fruit. (d, e) The difference in cell wall thickness between WT and OESlIMP3/ASSlIMP3 lines. The cell wall of fruit pericarp cells was observed with transmission electron microscope. The cell wall thickness was measured with ImageJ software. The data contain three replicates and SD was given by error bar. Scale bars, 1 μm. Statistical significance between WT and OESlIMP3/ASSlIMP3 lines was indicated by an asterisk (*P < 0.05, **P < 0.01).