Figure 1.

AMT1;1 D³⁵⁸N inhibits chlorophyll accumulation, NH₄ ⁺ uptake and rice resistance to ShB. (a) Leaves of the starter line for regeneration (Ds‐s) and mutant (m) were inoculated with R. solani AG1‐IA and photographed after infection for 48 h. (b) The lesion area on the leaves shown in (a) was analysed. Data represent the means standard error (SE) (n > 10). (c) Ds insertion was examined by Southern blot analysis. GUS DNA fragment was used as a probe. Ori. Ds indicates the original Ds insertion site in Ds‐s. (d) Leaf morphology of Ds‐s and m plants. (e) Chlorophyll content from Ds‐s and m plants was determined. (f) The mutation site of in OsAMT1;1 coding region was identified. The D³⁵⁸ was replaced with N in m plants. (g) AMT1;1 structure display, and position of D³⁵⁸ at the TM helix. (h) The NH₄ ⁺ content was calculated in Ds‐s and m plant roots. Significant differences at the P < 0.05 level are indicated by stars.