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. 2022 Feb 24;20(6):1085–1097. doi: 10.1111/pbi.13789

Figure 7.

Figure 7

Feedback activation of NH4 + transport via ethylene signalling is important for rice resistance to ShB. (a) NH4 +‐dependent expression levels ACO2, ACO3, EIN2, EIL1 and ERFs were verified by qRT‐PCR with NH4 + treatment for 3 hours. (b) Positive ethylene signalling regulators EIL1 and EIL2 and (c) negative ethylene signalling regulators ERS1 and ETR2 expression levels were monitored in wild‐type (DJ), AMT1;1 OX and AMT1 RNAi plants grown in LN conditions for 14 days. (d) NH4 +‐induced AMT1;1 and AMT1;2 expression levels in WT and EIL1 mutant (eil1) was monitored. Sample mRNA levels were normalized to those of Ubiquitin mRNA. Error bars represent means ± SE (n = 3). (e) Endogenous NH4 + levels in WT and eil1 were measured in roots grown in 0.5 × MS for 3 days. Error bars represent means ± SE (n = 20). Significant differences at the P < 0.05 level are indicated by different letters.