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(A) Primer extension analysis of the transcription start sites of the ppk gene. P. aeruginosa 8830 total RNA was annealed to oligonucleotide P1 and extended with murine reverse transcriptase. Lanes G, A, T, and C represent the dideoxy sequencing ladder carried out with the same primer. Lane 1, primer extension products. (B) Comparison of −35 and −10 motifs identified upstream of the transcription start site (the C residue that gave the most intense band) with ςE consensus sequences.
