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. 2022 May 23;221(7):e202111125. doi: 10.1083/jcb.202111125

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© 2022 Wang et al.

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Figure S4. — SMGL-1 resides in the ER-Golgi intermediate compartment and neighboring RAB-8-positive structures. (A–E) In smgl-1(RNAi) animals or smgl-1(ycxEx1659) mutants, the expression levels of GFP::SMGL-1 and endogenous SMGL-1 were decreased. (F and G) The intracellular juxtaposition between AMAN-2 and ILE-1 or GOLG-4. (H and I) Endogenous RAB-8 colocalized with MC::SMGL-1. (J and K) SMGL-1-positive puncta were separable from RAB-5, LAAT-1, and RME-1. Pearson’s correlation coefficients for GFP and mCherry signals were calculated (n = 12 animals). The signals from the apical membrane were avoided by manual ROI selection. Scale bars: 10 μm. Colored asterisks indicate intestinal lumen. Source data are available for this figure: SourceData FS4.