Video 1.
Reconstitution of branching MT nucleation using purified native augmin–γ-TuRC complex. Branching MT nucleation both from GMPCPP seeds (tracked by arrows) and from solution (tracked by arrowhead) in the presence of native augmin–γ-TuRC complex (10 nM augmin; 0.35 nM γ-TuRC) purified from nocodazole-arrested mitotic HAUS8-GFP-Strep knock-in HeLa cells. Images were collected with a TIRF microscope at 4-s intervals. Video is sped up 160 times (40 frames/s). Time is shown in the format min:s. Scale bars, 5 μm.