Video 10.

NEDD1 WD40 domain is essential for branching nucleation. Branching MT nucleation in the presence of 10 nM augmin-SNAP (blue) together with 10 nM γ-TuRC-GFP^StN or indicated concentrations of γ-TuRC^ΔWD/StN-GFP (green), all in phosphorylated forms. These complexes were separately purified from nocodazole-arrested mitotic HEK293T cells in the presence of coexpressed untagged CDK1/cyclin B and HA-PLK1 (augmin^CDK1/PLK1, γ-TuRC^{StN/CDK1/PLK1}, and γ-TuRC^{ΔWD/StN/CDK1/PLK1}). Images were collected with a TIRF microscope at 4-s intervals. Video is sped up 160 times (40 frames/s). Time is shown in the format min:s. Scale bars, 5 μm.

https://movie.rupress.org/media/by_doi/10.1083/jcb.202109053.v10/source