Video 5.
MT nucleation at different γ-TuRC concentrations. MT nucleation in the presence of indicated concentrations of γ-TuRC -GFP (green). Each arrow indicates an MT minus end capped by a γ-TuRC-GFP spot. Images were collected with a TIRF microscope at 2-s intervals. Video is sped up 80 times (40 frames/s). Time is shown in the format min:s. Scale bars, 5 μm.