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. 2022 May 13;15:100289. doi: 10.1016/j.mtbio.2022.100289

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 1 — Characterization and intercellular uptake of IC/IR820 NPs. (A) UV–vis absorption spectra of IC/IR820 NPs. (B) TEM image of IC/IR820 NPs. Scale bar: 100 nm. (C) Size distribution of IC/IR820 NPs. (D) Photothermal activity of IC/IR820 NPs dispersed in water at various concentrations (λ = 808 nm, P = 1.0 W/cm²; irradiation time = 5 min). (E) Infrared thermal images of IR820 and IC/IR820 NPs in water after laser irradiation (λ = 808 nm, P = 1.0 W/cm²; irradiation time = 5 min). (F) Fluorescence microscopy images and (G) flow cytometric results of cell uptake of IC/IR820 NPs into MDA-MB-231 cells at different time points. Scale bar: 20 μm. (H) Fluorescence microscopy images and (I) flow cytometric results of cell uptake of IR820 and IC/IR820 nanoparticles in MDA-MB-231 cells at different concentrations after incubation for 4 h. Scale bar: 20 μm.