The anti-breast cancer effect of IC/IR820 NPs. (A–C) Viability of MDA-MB-231, MCF-7, and 4T1 cells co-cultured with IC, IR820, and IC/IR820 nanoparticles. (λ = 808 nm, P = 1.0 W/cm2; irradiation time = 20 s). (D–G) Representative images for colony formation of MDA-MB-231, MCF-7, and 4T1 cells co-cultured with IC, IR820, and IC/IR820 nanoparticles. ∗∗∗P < 0.001. (λ = 808 nm, P = 1.0 W/cm2; irradiation time = 30 s). (H–K) Quantification of MDA-MB-231, MCF-7, and 4T1 cells co-cultured with IC, IR820, and IC/IR820 nanoparticles in the EDU assay. Scale bar: 20 μm. (λ = 808 nm, P = 1.0 W/cm2; irradiation time = 20 s). (L–N) LDH assay of MDA-MB-231, MCF-7, and 4T1 cells co-cultured with IC, IR820, and IC/IR820 nanoparticles. (λ = 808 nm, P = 1.0 W/cm2; irradiation time = 20 s).