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. 2022 May 13;15:100289. doi: 10.1016/j.mtbio.2022.100289

Fig. 3.

Fig. 3

IC/IR820 NPs-mediated ROS induce breast cancer cell death. (A–C) Immunofluorescence images to show subcellular localization of IR820 and IC/IR820 NPs. Scale bar: 20 ​μm. (D) Fluorescence microscopy images and (E) flow cytometry analysis for intracellular ROS generation of MDA-MB-231 ​cells using DCFH-DA as a probe. Scale bar: 20 ​μm. (λ ​= ​808 ​nm, P ​= ​1.0 ​W/cm2; irradiation time ​= ​2 ​min). (F–H) Flow cytometry statistical analysis for intracellular ROS generation of MDA-MB-231, MCF-7 and 4T1 cells using DCFH-DA as a probe. Scale bar: 20 ​μm. (λ ​= ​808 ​nm, P ​= ​1.0 ​W/cm2; irradiation time ​= ​2 ​min). (I–K) Viability of specific groups with or without NAC treatment in MDA-MB-231, MCF-7 and 4T1 cells. (λ ​= ​808 ​nm, P ​= ​1.0 ​W/cm2; irradiation time ​= ​20 ​s).