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. 2022 May 13;15:100289. doi: 10.1016/j.mtbio.2022.100289

Fig. 4.

Fig. 4

IC/IR820 NPs trigger ROS-dependent apoptosis in breast cancer cells. (A–D) Apoptosis of IR820, IC, and IC/IR820 NPs were evaluated by annexin V-FITC/PI staining in MDA-MB-231, MCF-7 and 4T1 cells, quantification of apoptotic cell ratio. (λ ​= ​808 ​nm, P ​= ​1.0 ​W/cm2; the irradiation time ​= ​2 ​min). (E–G) Immunoblot analysis of apoptotic markers for MDA-MB-231, MCF-7 and 4T1 cells treated IC, IR820, IC/IR820 with or without NAC treatment. (λ ​= ​808 ​nm, P ​= ​1.0 ​W/cm2; irradiation time ​= ​2 ​min). (H–J) Viability of specific groups with or without ZVAD treatment in MDA-MB-231, MCF-7 and 4T1 cells. (λ ​= ​808 ​nm, P ​= ​1.0 ​W/cm2; irradiation time ​= ​20 ​s).