Fig. 7.

IC/IR820 NPs inhibit breast cancer metastasis. (A–B) Immunoblot analysis of EMT markers for MDA-MB-231 and MCF-7 ​cells treated with IC, IR820, IC/IR820 with or without NAC treatment/irradiation (808 ​nm, 1.0 ​W ​cm⁻², 20 ​s). (C–E) Representative images of migration, invasion, and scratch test of MDA-MB-231 ​cells, quantification of migration/invasion cell number and migratory distance. Scale bar for migration and invasion assay: 50 ​μm. Scale bar for scratch test: 20 ​μm. (F–I) Representative lung, liver H&E staining of mice bearing 4T1 orthotopic breast tumor xenografts treated with vehicle, IR820 ​+ ​Laser, IC, IC/IR820 NPs ​+ ​Laser at the end of the treatment period. Quantification of the relative metastatic area in lungs and metastatic nodes in livers. Scale bar: 50 ​μm.