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. 2022 May 24;13:2894. doi: 10.1038/s41467-022-30588-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Conceptual diagram representing the cloning of eGFP into a lentiviral plasmid backbone. b QUEEN operational flow representing the same DNA cloning process. The visualization was directly generated from the output gbk file using QUEEN. The file shape, round, and uncolored rectangular objects represent the input gbk files, dsDNA objects, and feature objects, respectively. Colored boxes represent QUEEN operational functions with the colors corresponding to Fig. 2. Open and closed circle-headed lines represent information flows as QUEEN objects and input parameters, respectively.