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. 2022 May 24;13:2894. doi: 10.1038/s41467-022-30588-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The construction lineages of base editor plasmids with recycling of their DNA sequences. The plasmid names with gray, yellow, and white boxes represent those obtained from Addgene, newly synthesized, or described by QUEEN in this study, respectively. b Recovery of the “Cas9(D10A)” feature after joining two fragments each from a different DNA object having the same feature.