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. 2022 May 24;13:2894. doi: 10.1038/s41467-022-30588-x

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a The six-input, one-output Boolean logic LUT of the BLADE system. Left, site-specific DNA recombinases (round nodes) and their corresponding target DNA sites (round rectangles). Right, the genetic Boolean logic LUT circuit. b The wiring diagram of the genetic circuit. c Annotated DNA sequence outcomes of each signal input pattern simulated by QUEEN. GFP expresses if one of the GFP-encoding genes is placed in the same direction as the upstream promoter without being prevented by a transcription terminator.