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. 2022 May 24;8:62. doi: 10.1038/s41531-022-00327-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a The workflow of sampling, sequencing and metagenome-assembled genomes (MAGs) binning of gut microbiota and serum neurotransmitters, all elements in this image were the graph of results of this article or created by in iPad. b Statistical differences in the clinical parameters within and between groups were evaluated with paired t-tests and t-tests, respectively. Significant p values between sample pairs are shown. Boxplot elements: center line, median; box limits, upper and lower quartiles; whiskers, 1.5x interquartile range; points, outliers. Each dot represents a data point of a participant, and data of the same participant at different time points are connected by straight lines. “Pro” and “Pla” represent the Probio-M8 group and Placebo group, respectively. “0d”, “1 M”, and “3 M” represent the baseline before the intervention, 1 and 3 months after intervention, respectively. UPDRS-III, MMSE, PDSS, HAMA, HAMD-17, PAC-QOL represent Unified PD Rating Scale-III, Mini-mental State Examination, Parkinson’s Disease Sleep Scale, Hamilton Anxiety Scale, Hamilton Depression Scale-17, Patient-Assessment of Constipation Quality of Life, respectively.