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. 2022 May 24;13:2899. doi: 10.1038/s41467-022-30625-9

Fig. 6. Ano1 knockout in osteoclast inhibits OVX-induced bone loss.

Fig. 6

a Western blot analysis of ANO1 protein level in human bone specimens from two groups (left). The quantification of ANO1 protein level in human bone specimens from two groups (right). Non-osteoporosis group (T-score > −2.5), n = 6, and Osteoporosis group (T-score ≤ −2.5), n = 6. b Correlation analysis between ANO1 mRNA level and NFATc1, ACP5, CTSK and MMP9 mRNA levels in human bone specimens (n = 32). c Correlation analysis between ANO1 mRNA level in bone tissues and β-CTX level in human serum (n = 32). d Western blot analysis of Ano1 protein level in bone tissues with Sham or ovariectomized (OVX) treatment (left). The quantification of Ano1 protein level in bone tissues with Sham or OVX treatment (right) (n = 3). e Representative images showing three-dimensional trabecular architecture as determined by micro-CT reconstruction of the distal femurs from the groups of mice indicated. Representative images of six independent tissue in each group. Scale bar, 0.5 mm. f Micro-CT measurements for BV/TV, Tb.N, Tb.Th and Tb.Sp in the distal femurs. n = 6 for each group. g Representative images of TRAP staining of the proximal tibia. Scale bar, 100 μm. n = 6 for each group. h Histomorphometry analysis of the images for number of osteoclasts per bone perimeter (N.Oc/B.Pm) and osteoclast surface per bone surface (Oc.S/BS). n = 6 for each group. i ELISA analysis of CTX-1 protein level in serum. n = 6 for each group. j QRT-PCR analysis of NFATc1, Acp5, Ctsk, and Mmp9 mRNA levels in bone tissues. n = 6 for each group. All data are the mean ± s.e.m. Two-tailed unpaired Student’s t-test was used for statistical evaluations of two group comparisons. Statistical analysis with more than two groups was performed with two-way analysis of variance (ANOVA) with Šídák post-hoc test to determine group differences. Source data are provided as a Source Data file.