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. 2022 May 24;13:2882. doi: 10.1038/s41467-022-30570-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a N-terminal signal sequences tested on HsMOR. b Effect of signal sequences on HsMOR biosensor sensitivity to DAMGO and morphine. Unpaired one-way ANOVA of pEC₅₀s: n = 3 biologically independent experiments, >7595 cells/strain/replicate; P < 0.0001 for each agonist; Dunnett’s multiple comparisons tests against WT shown. c Effect of signal sequences on HsMOR signaling population. Unpaired one-way ANOVA: n = 3 biologically independent experiments, >7595 cells/strain/replicate; P < 0.0001 (DAMGO) and P < 0.001 (morphine); Dunnett’s multiple comparisons tests against WT shown. d Imaging of HsMOR-GFP with the indicated signal sequences in a cholesterol-rich background with the ER marker RFP-SCS2(tmh). n = 3 biologically independent experiments. Data presented as mean +/− SEM. Scale bar is 2 μm. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Source data are provided as a Source Data file.