Fig. 3.

Comparison between the effects of levetiracetam (LEV, in all IHKA mice) and chemogenetic suppression (clozapine in DREADD-expressing IHKA mice). The number of seizures per hour (A) and the seizure length (B) are plotted in 30-min bins for LEV (800 mg/kg) and clozapine (0.1 mg/kg) during 8 h before and 8 h after treatments. Levetiracetam treatment only affected the number of seizures during the first 30 min after treatment and between 1.5 and 2 h post injection (A) and did not affect the length of seizures (B). Chemogenetic suppression with clozapine suppressed the number of seizures during all 30-min bins after treatment (A) as well as seizure length during multiple post-treatment bins (B). Asterisk indicates significant differences between post-treatment intervals and baseline (*p < 0.05, ***p < 0.001). The arrow indicates the administration of clozapine or LEV. No seizures were present during the first 30 min after clozapine treatment; therefore, no statistics could be performed on the seizure length during this bin (B)