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. 1999 May;65(5):2103–2111. doi: 10.1128/aem.65.5.2103-2111.1999

FIG. 6.

FIG. 6

MNP, LIP, and laccase isozymes separated by IEF. The cultures of F. flavus were grown in LN medium for 9 days, and the extracellular fluid was used for IEF. The gels were stained with 3,3′-diaminobenzidine in the presence of MnSO4 and H2O2 for MNP (A) (2), o-dianisidine and H2O2 for LIP (B) (30), and ABTS without H2O2 for laccase (C) (34). The numbers on the left are the pI values.