Fig. 4.
Cy5-labeled D-2PMPA is targeted to macrophages in SOD1G93A gastrocnemius muscle. A GCPII activity in gastrocnemius muscles excised from WT and SOD1G93A mice treated with vehicle or D-2PMPA for 2 weeks. Bars represent mean ± SEM. Black bars represent WT values. Blue bars represent SOD1G93A vehicle–treated mouse values. Red bars represent SOD1G93A D-2PMPA–treated mouse values. ***p < 0.001. n = 5/group. B, D Representative images from gastrocnemius muscles in WT mice showing no Cy5-D-2PMPA staining (B) localizing with CD68 + macrophages (C). Merged image shown in D with nuclei stained blue. E, G Representative images from gastrocnemius muscles in SOD1G93A mice showing Cy5-D-2PMPA staining (B) localizing with CD68 + macrophages (C). Merged image shown in D with nuclei stained blue. Scale bar, 10 µm