Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Purification of recombined HN protein
| Fraction | Volume (ml) | Protein (mg) | Activity neuraminidase (unit) | Specific activity (pmol/min/mg) | Fold purification | Yield % |
|---|---|---|---|---|---|---|
| Cultural supernatant | 200 | 40.6 | 63.34 | 1.5 | 1× | 100 |
| Pooled eluates from SP | 20 | 29.15 | 62.66 | 2.14 | 1.4× | 98 |
| Pooled eluates from Ni-NTA | 20 | 10.64 | 61.84 | 5.8 | 3.8× | 97 |
*Specific activity = (activity enzyme/protein)
**Fold purification = (specific activity/starting special activity)
***Yield = [(activity enzyme/starting activity enzyme) × 100]
