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Primer sequences of genes
| mRNA | Oligonucleotide Primers (5′–3′) |
|---|---|
| GAPDH |
F-ATGGGAAGCTGGTCATCAAC R-GGATGCAGGGATGATGTTCT |
| Runx1 |
F-CTCTCAGCGGAACTTTCCAGTC′ R-TGACAGGAGGCGAGTAGGTGAA |
| Sox 9 |
F-AGGAAGCTGGCAGACCAGTA R-CGGCAGGTATTGGTCAAACT |
| Aggrecan |
F-TGGCATTGAGGACAGCGAAG R-TCCAGTGTGTAGCGTGTGGAAATAG |
| COL2a1 |
F-CGAGGTGACAAAGGAGAAGC R-CTGGTTGTTCAGCGACTTGA |
