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High speed centrifugation successfully concentrated conditioned media. A Schematic of macrophage-derived concentrated CM (CCM) preparation and implantation of CCM-embedded alginate patch into skin wound model. B Proteomic microarray of cytokines contained in supernatant, 10% serum, CM and CCM, respectively. No signals detected in the supernatant. C ELISA analysis of angiogenic growth factors (bFGF and VEGF) contained in two different types of CM and CCM. (*p < 0.05, ****p < 0.0001)
