TABLE 2.
PTS activity of S. aureus extracts complemented with L. monocytogenes Scott A HPr
| Reaction componentsa | PTS activityb |
|---|---|
| HPr (wild type) | 316 (100) |
| HPr (wild type) + FDP | 148 (47) |
| H15A | 4 (1) |
| H15D | 12 (4) |
| S46T | 235 (74) |
| S46T + FDP | 127 (40) |
| S46D | 117 (37) |
| S46D + FDP | 75 (24) |
a
All reaction mixtures contained S. aureus S797A extracts, 5 mM PEP, 5 mM MgCl2, 1 mM ATP, 0.2 mM o-nitrophenyl-β-d-galactopyranoside (ONPG), and 2 ng of L. monocytogenes HPr (from the wild type or mutant forms). FDP (20 mM) was added where indicated.
b
Activities (from duplicate samples) are expressed as micromoles of o-nitrophenyl formed per minute per milligram of HPr protein (and as a percent of the wild-type control). Control reaction mixtures containing no HPr had no measurable PTS activity.