FIGURE 4.
Schwann cells of the sciatic nerve express sortilin. (A) Double-labeled immunofluorescence microscopy of sortilin (green) and s100β (red) in longitudinal sections of wild-type (WT) naïve sciatic nerve. Nuclei are labeled with Hoechst (blue). White arrows demonstrate Schwann cells expressing sortilin. Scale bar 50 μm. (B) Double-label immunofluorescence microscopy of sortilin (green) and s100β (red; Schwann cell marker) in longitudinal sections of naïve sciatic nerves and 5 days after crush injury in WT and Sort1–/– distal sciatic nerves. Nuclei are labeled with Hoechst (blue). The white arrow points to a sortilin-positive (green) s100β-positive (red) WT Schwann cell. Scale bar 100 μm upper images and 50 μm lower images.